Case ID: M24-007P^

Published: 2024-05-23 09:49:32

Last Updated: 1717513583


Peter Wiktor
Abhik Manna
Petra Fromme
Alexandra Ros

Technology categories

Advanced Materials/NanotechnologyBioanalytical Assays, Chemistries & DevicesPhysical Science

Licensing Contacts

Physical Sciences Team

Nozzle and Dispenser for Time-Resolved Electron Microscopy Structural Studies


In the realm of electron microscopy, there is a burgeoning interest in investigating dynamic processes like protein folding and molecular interactions in real time. However, traditional methods mostly yield static images, which makes it difficult to investigate fast-moving dynamic occurrences. One of the main challenges is that protein samples must be quickly combined with substrates before being placed on electron microscopy grids. Current mixing techniques are limited in terms of simplicity, speed, and accuracy. The swift and accurate mixing of substrates with protein samples is the main difficulty in time-resolved electron microscopy investigations. For the purpose of starting chemical processes or changing the structure of proteins, this step is essential. Present techniques, including angle dispensing and microfluidic mixers, are neither fast nor efficient enough to efficiently capture short-lived reaction intermediates.

Invention Description

Researchers at Arizona State University have developed a novel method for time-resolved electron microscopy that integrates the mixing process directly into the dispensing nozzle. Unlike traditional approaches, this technique enables faster mixing of substrates with protein samples inside the nozzle itself, allowing for shorter experiment time points. This technology offers the potential for greater advancements in knowledge of reaction processes and protein structure that were previously inaccessible with conventional methods. Through the utilization of this nozzle and dispenser system, dynamic processes with previously unattainable temporal resolution can be investigated, as it overcomes the constraints of current mixing approaches. With the potential to speed advances in molecular biology and medication development as well as provide light on basic biological processes, this discovery has far-reaching ramifications.

Potential Applications

  • Biomedical research
  • Pharmaceutical development
  • Materials science research

Benefits and Advantages

  • Enhanced temporal resolution
  • Streamlined workflow
  • Improved accuracy
  • Cost efficiency